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Lent is a time of reflection, conversion, and getting closer to God. To allow for special prayer during these days of preparation, three thematic chapels featuring international art masterpieces pertaining to the Passion of Christwill remain open: the Piedad Altar, Santa Ana Chapel, and the Jácomes Chapel.

These chapels can be visited during opening hours of the Cathedral, from 8:00 to 17:00. From 8:00 to 10:30 access will be through the San Miguel and Los Palos gates and, from 10:45 to 17:00, through the authorized cultural visit doors: San Cristóbal and Lagarto.

The Jácomes Chapel is dedicated to the Virgen de las Angustias, and there resides a baroque altarpiece presided over by a picture of the Virgen de la Piedad de Juan de Roelas from 1609. Prominent in the piece are the figures of the Virgen with her son in her lap, the head of Christ above her surrounded by angels, with two stained glass windows at her side, and two images of angels. The gate decorated with plaster and a grating embossed with noble shields. At the sides of the chapter hang the pictures of Jesus the Nazarene and San Pedro the penitent.

In the Santa Ana Chapel hangs a magnificent painting dating back to around 1560 that depicts Christ Crucified receiving the devotion of Santo Cristo de Maracaibo, in which the background landscape, one of the most beautiful in Sevillian painting, shows that the painter had knowledge of Raphael.

Notable at the Altar de la Piedad is the painting of the main scene of La Piedad. It centers on the dead body of Christ after having come down from the Cross, carried by Saint John the Evangelist while the Virgin Mary stays, shaken, by his side accompanied by the holy saints.